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celltracker tm orange cmtmr dye  (Thermo Fisher)


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    Thermo Fisher celltracker tm orange cmtmr dye
    Celltracker Tm Orange Cmtmr Dye, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/celltracker+tm+orange+cmtmr+dye/bio_rxiv__2025__01__20__633914-41-10-15
    Average 90 stars, based on 1 article reviews
    celltracker tm orange cmtmr dye - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    other:

    Article Title: Microfluidics sorting enables the isolation of an intact cellular pair complex of CD8 + T cells and antigen-presenting cells in a cognate antigen recognition-dependent manner
    Article Snippet: OVA-H2K b -BW5147 cells, H2K b -BW5147 cells, OT-I splenocytes, and C57BL/6 splenocytes were stained for 30 min at 37°C using 20 μM of CMTMR (CellTracker TM Orange CMTMR Dye, Thermo Fisher Scientific), 50 μM of CMAC (CellTracker TM Blue CMAC Dye, Thermo Fisher Scientific), 1 μM of CFSE (CellTrace TM CFSE Cell Proliferation Kit, Thermo Fisher Scientific), and 2 μM of Far Red (CellTrace TM Far Red Cell Proliferation Kit, Thermo Fisher Scientific), respectively.

    Labeling:

    Article Title: Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell function
    Article Snippet: HUVECs were labeled with 1 μM CellTracker TM Deep Red Dye (Life Technologies) in PBS for 20min at 37°C and 4×10 4 labeled cells were seeded in co-culture with pCAFs. .. After 24h of co-culture, 8.5×10 3 THP-1 cells, which were labeled with 2 μM CellTracker TM Orange CMTMR Dye (Life Technologies) in PBS for 20min at 37°C, were added in each well in M199 medium (Life Technologies) supplemented with 10% FBS. .. After 45min, unbound THP-1 cells were removed by three washes in 1% BSA in PBS with calcium and magnesium (Sigma-Aldrich).

    Confocal:

    Article Title: SLAMF6 enables efficient attachment, synapse formation, and killing of HIV-1-infected CD4 + T cells by virus-specific CD8 + T cells
    Article Snippet: For flow cytometry CellTrace CFSE Cell Proliferation Kit (Life Science), CellTracker Violet BMQC Dye (Life Science), and PKH26 Red Fluorescent Cell dye TM (Sigma Aldrich) were used. .. For confocal microscopy analysis CellTracker TM Blue CMF2HC dye and CellTracker TM Orange CMTMR dye (Invitrogen) were used. .. Synthetic peptides were purchased from the MGH peptide core or Genscript as follows: HIV-A*02-SL9, SLYNTVATL; HIV-B*57-KF11, KAFSPEVIPMF; Influenza-A*02-GL9 GILGFVFTL; Epitope identification was facilitated by reference to the Immune Epitope Database ( www.iedb.org ).

    Microscopy:

    Article Title: SLAMF6 enables efficient attachment, synapse formation, and killing of HIV-1-infected CD4 + T cells by virus-specific CD8 + T cells
    Article Snippet: For flow cytometry CellTrace CFSE Cell Proliferation Kit (Life Science), CellTracker Violet BMQC Dye (Life Science), and PKH26 Red Fluorescent Cell dye TM (Sigma Aldrich) were used. .. For confocal microscopy analysis CellTracker TM Blue CMF2HC dye and CellTracker TM Orange CMTMR dye (Invitrogen) were used. .. Synthetic peptides were purchased from the MGH peptide core or Genscript as follows: HIV-A*02-SL9, SLYNTVATL; HIV-B*57-KF11, KAFSPEVIPMF; Influenza-A*02-GL9 GILGFVFTL; Epitope identification was facilitated by reference to the Immune Epitope Database ( www.iedb.org ).

    Analysis:

    Article Title: SLAMF6 enables efficient attachment, synapse formation, and killing of HIV-1-infected CD4 + T cells by virus-specific CD8 + T cells
    Article Snippet: For flow cytometry CellTrace CFSE Cell Proliferation Kit (Life Science), CellTracker Violet BMQC Dye (Life Science), and PKH26 Red Fluorescent Cell dye TM (Sigma Aldrich) were used. .. For confocal microscopy analysis CellTracker TM Blue CMF2HC dye and CellTracker TM Orange CMTMR dye (Invitrogen) were used. .. Synthetic peptides were purchased from the MGH peptide core or Genscript as follows: HIV-A*02-SL9, SLYNTVATL; HIV-B*57-KF11, KAFSPEVIPMF; Influenza-A*02-GL9 GILGFVFTL; Epitope identification was facilitated by reference to the Immune Epitope Database ( www.iedb.org ).



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    celltracker tm orange cmtmr dye  (Thermo Fisher)
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    Interaction of H. influenzae strains with 16HBE14 human bronchial epithelial cells. (A) Numbers of total tissue cell adherent bacterial colony-forming units (CFU) 4 or 24 h post-infection with Hi2019 WT Hi2019 Δ dmsA or Hi2019 Δ dmsAc . (B) Bacterial intracellular CFU at 4 and 24 h post-infection. (C) Fluorescence microscopy of 16HBE14 tissue cells infected with Hi2019 WT or Hi2019 Δ dmsA . 16HE14 cells are stained with <t>CellTracker</t> orange; Hi2019 appears in green following antibody-mediated detection. While at 4 h post-infection both strains show similar colonization patterns, after 24 h growth of Hi2019 Δ dmsA shows a different pattern with less even colonization of the tissue cells. Images are representative of three independent experiments. Statistical testing (A,B) used unpaired, two-tailed t -tests, ∗∗ p < 0.01, ∗∗∗ p < 0.001, **** p < 0.0001.
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    celltracker tm orange dye cmtmr 1 μm  (Thermo Fisher)
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    Interaction of H. influenzae strains with 16HBE14 human bronchial epithelial cells. (A) Numbers of total tissue cell adherent bacterial colony-forming units (CFU) 4 or 24 h post-infection with Hi2019 WT Hi2019 Δ dmsA or Hi2019 Δ dmsAc . (B) Bacterial intracellular CFU at 4 and 24 h post-infection. (C) Fluorescence microscopy of 16HBE14 tissue cells infected with Hi2019 WT or Hi2019 Δ dmsA . 16HE14 cells are stained with <t>CellTracker</t> orange; Hi2019 appears in green following antibody-mediated detection. While at 4 h post-infection both strains show similar colonization patterns, after 24 h growth of Hi2019 Δ dmsA shows a different pattern with less even colonization of the tissue cells. Images are representative of three independent experiments. Statistical testing (A,B) used unpaired, two-tailed t -tests, ∗∗ p < 0.01, ∗∗∗ p < 0.001, **** p < 0.0001.
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    Image Search Results


    Interaction of H. influenzae strains with 16HBE14 human bronchial epithelial cells. (A) Numbers of total tissue cell adherent bacterial colony-forming units (CFU) 4 or 24 h post-infection with Hi2019 WT Hi2019 Δ dmsA or Hi2019 Δ dmsAc . (B) Bacterial intracellular CFU at 4 and 24 h post-infection. (C) Fluorescence microscopy of 16HBE14 tissue cells infected with Hi2019 WT or Hi2019 Δ dmsA . 16HE14 cells are stained with CellTracker orange; Hi2019 appears in green following antibody-mediated detection. While at 4 h post-infection both strains show similar colonization patterns, after 24 h growth of Hi2019 Δ dmsA shows a different pattern with less even colonization of the tissue cells. Images are representative of three independent experiments. Statistical testing (A,B) used unpaired, two-tailed t -tests, ∗∗ p < 0.01, ∗∗∗ p < 0.001, **** p < 0.0001.

    Journal: Frontiers in Microbiology

    Article Title: The DmsABC Sulfoxide Reductase Supports Virulence in Non-typeable Haemophilus influenzae

    doi: 10.3389/fmicb.2021.686833

    Figure Lengend Snippet: Interaction of H. influenzae strains with 16HBE14 human bronchial epithelial cells. (A) Numbers of total tissue cell adherent bacterial colony-forming units (CFU) 4 or 24 h post-infection with Hi2019 WT Hi2019 Δ dmsA or Hi2019 Δ dmsAc . (B) Bacterial intracellular CFU at 4 and 24 h post-infection. (C) Fluorescence microscopy of 16HBE14 tissue cells infected with Hi2019 WT or Hi2019 Δ dmsA . 16HE14 cells are stained with CellTracker orange; Hi2019 appears in green following antibody-mediated detection. While at 4 h post-infection both strains show similar colonization patterns, after 24 h growth of Hi2019 Δ dmsA shows a different pattern with less even colonization of the tissue cells. Images are representative of three independent experiments. Statistical testing (A,B) used unpaired, two-tailed t -tests, ∗∗ p < 0.01, ∗∗∗ p < 0.001, **** p < 0.0001.

    Article Snippet: Epithelial cells were stained with CellTracker TM Orange CMTMR fluorescent dye (Thermo Fisher; 200 μl of 1 μg/ml solution) for 1 h at room temperature in the dark before coverslips were mounted onto slides using ProLong ® Gold Antifade Reagent (Thermo Fisher).

    Techniques: Infection, Fluorescence, Microscopy, Staining, Two Tailed Test